Fast DNA arrays oligonucleotides design with OliGO!©
Brief info

OliGO!© selects the most suitable oligonucleotides to be used as probes in DNA arrays experiments by:

  1. Choosing the most especific sequences in each gene (using the BLAST algorithm with optimized parameters).

  2. Selecting sequences with homogeneous melting temperatures (using a Nearest-neighbour method based algorithm).

  3. Discarding oligonucleotides with high probability to have secondary structure (predicting oligonucleotide self homology by BLAST).


More

All presented on an interactive CD
(no software installations, no minimum requirements, just insert CD and visualize!)
which includes:

  • Your complete list of oligonucleotides in both tabulated and Fasta formats.

  • A page showing all relevant information about each oligo (specificity, melting temperature, self-homology prediction, target vs oligo hybridization diagram...).

You may also want to download our pdf documentation, describing OliGO!'s most powerful features:




Algorithm references:

BLAST: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997).
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
Nearest-Neighbour: SantaLucia, J., Jr (1998). A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci U S A. 95(4):1460-5.


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