TMpred output for RHI00001

[ISREC-Server] Date: Tue Oct 30 13:51:44 MET 2001


Sequence: MNL...QEA, length: 599
Prediction parameters: TM-helix length between 14 and 35

1.) Possible transmembrane helices

The sequence positions in brackets denominate the core region.
Only scores above 500 are considered significant.
Inside to outside helices :   3 found
      from        to    score center
  74 (  74)  92 (  89)    309     82
 211 ( 211) 235 ( 228)    310    221
 353 ( 355) 371 ( 371)    704    363

Outside to inside helices :   2 found
      from        to    score center
  74 (  74)  92 (  92)    258     82
 355 ( 357) 374 ( 372)    784    364


2.) Table of correspondences

Here is shown, which of the inside->outside helices correspond to which of the outside->inside helices.
Helices shown in brackets are considered insignificant.
A "+"-symbol indicates a preference of this orientation.
A "++"-symbol indicates a strong preference of this orientation.
 
           inside->outside | outside->inside
(   74-  92 (19)  309    ) |(   74-  92 (19)  258    ) 
(  211- 235 (25)  310 ++ ) |
   353- 371 (19)  704      |   355- 374 (20)  784      


3.) Suggested models for transmembrane topology

These suggestions are purely speculative and should be used with extreme caution since they are based on the assumption that all transmembrane helices have been found.
In most cases, the Correspondence Table shown above or the prediction plot that is also created should be used for the topology assignment of unknown proteins.
2 possible models considered, only significant TM-segments used

-----> slightly prefered model: N-terminus outside
 1 strong transmembrane helices, total score : 784
 # from   to length score orientation
 1  355  374 (20)     784 o-i

------> alternative model
 1 strong transmembrane helices, total score : 704
 # from   to length score orientation
 1  353  371 (19)     704 i-o


You can get the prediction graphics shown above in one of the following formats:


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